WB result of Lysozyme Rabbit pAb
Primary antibody: Lysozyme Rabbit pAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 15 kDa
Lysozyme Rabbit Polyclonal Antibody
Lysozyme Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Lysozyme |
| Synonyms | Lysozyme C; 1,4-beta-N-acetylmuramidase C; LZM; LYZ |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P61626 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms |
| Positive Sample | HL-60, THP-1, J774A.1 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms |
| IHC-P | 1:1000 | Hu, Ms |
Background
Lysozyme is a small, ubiquitous enzyme that catalyzes the hydrolysis of β-1,4-glycosidic linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine in bacterial peptidoglycan, thereby weakening the cell wall and leading to osmotic lysis; it is abundant in tears, saliva, egg white, and other secretions, serving as a front-line innate immune defense against Gram-positive bacteria, and has been extensively studied since Alexander Fleming’s isolation in 1922, becoming a model system for protein folding, enzyme kinetics, and X-ray crystallography while finding applications in food preservation, pharmaceuticals, and biotechnology.
Picture
Picture
Western Blot
WB result of Lysozyme Rabbit pAb
Primary antibody: Lysozyme Rabbit pAb at 1/1000 dilution
Lane 1: J774A.1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 15 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human small intestine. Anti-Lysozyme antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Lysozyme antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Lysozyme antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Lysozyme antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Lysozyme antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
