WB result of LRRK2 Recombinant Rabbit mAb
Primary antibody: LRRK2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: U-87MG whole cell lysate 20 µg
Lane 4: A172 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 286 kDa
Observed MW: 270 kDa
LRRK2 Recombinant Rabbit mAb (S-2484-164)
LRRK2 Recombinant Rabbit mAb (S-2484-164)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | LRRK2 |
| Synonyms | Leucine-rich repeat serine/threonine-protein kinase 2; Dardarin; PARK8 |
| Immunogen | Synthetic Peptide |
| Location | Lysosome, Endoplasmic reticulum membrane, Golgi apparatus membrane, Mitochondrion outer membrane |
| Accession | Q5S007 |
| Clone Number | S-2484-164 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A549, SH-SY5Y, U-87MG, A172, MEF, NIH/3T3, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
LRRK2 (leucine-rich repeat kinase 2) is a large, multidomain protein belonging to the ROCO family, featuring a Ras-of-complex (ROC) GTPase domain followed by a C-terminal-of-ROC (COR) domain and a serine/threonine kinase domain, along with armadillo, ankyrin, leucine-rich repeat and WD40 repeat regions that collectively mediate protein–protein interactions, membrane association and autoregulation, and whose pathogenic mutations—most notably G2019S in the kinase domain—are the commonest known genetic cause of both familial and sporadic Parkinson’s disease by promoting hyper-kinase activity, mitochondrial fragmentation, autophagic-lysosomal dysfunction and neuronal toxicity.
Picture
Picture
Western Blot
WB result of LRRK2 Recombinant Rabbit mAb
Primary antibody: LRRK2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MEF whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 286 kDa
Observed MW: 270 kDa
WB result of LRRK2 Recombinant Rabbit mAb
Primary antibody: LRRK2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 286 kDa
Observed MW: 270 kDa
