Measured in a serum-free cell proliferation assay using human MCF-7 cells. The EC50 for this effect is 0.87-2.0 ng/mL.
LR3-IGF-I Protein, Human/Porcine/Bovine
LR3-IGF-I Protein, Human/Porcine/Bovine
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | LR3 Insulin-like Growth Factor-I, MGF, IGFI, IGF1A, IGF, IBP1 |
| Accession | P05019 |
| Amino Acid Sequence | Gly49-Ala118 (Glu51Arg) |
| Expression System | E.coli |
| Molecular Weight | 10 kDa (Reducing) |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 50mM HAc |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Endocrinology. 1999 Oct;140(10):4699-705. |
Background
IGF-I is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, insulin-like, mitogenic activities. IGF-I is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-II, which is also a major fetal growth factor. Human Long R3 Insulin-like Growth Factor-I (rhLR3-IGF-I) contains an 83 amino acid analog of human IGF-I. Compared to the complete human IGF-I sequence, an addition of the rhLR3-IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3) and a13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of human Long R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.
Picture
Picture
Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
