LPCAT1 Recombinant Rabbit mAb (S-6053)

Catalog Number: S0B60501 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	LPCAT1
Synonyms	Lysophosphatidylcholine acyltransferase 1; LPC acyltransferase 1; LPCAT-1; LysoPC acyltransferase 1; 1-acylglycerol-3-phosphate O-acyltransferase; 1-acylglycerophosphocholine O-acyltransferase; 1-alkenylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase (Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT); AYTL2; PFAAP3
Location	Cytoplasm, Cell membrane, Endoplasmic reticulum
Accession	Q8NF37
Clone Number	S-6053
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	A431, A549, PC-3
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu
IHC-P	1:2000	Hu

Background

LPCAT1 (Lysophosphatidylcholine Acyltransferase 1) is a membrane-bound enzyme primarily localized to the endoplasmic reticulum and Golgi apparatus, which catalyzes the acyltransferase reaction between lysophosphatidylcholine (LPC) and acyl-CoA, incorporating unsaturated fatty acids (such as arachidonic acid) into the sn-2 position of phosphatidylcholine (PC). It plays a critical role in the synthesis of pulmonary surfactant (whose major component is dipalmitoylphosphatidylcholine) and is an essential enzyme for maintaining normal lung compliance and respiratory function. However, in recent years, accumulating evidence has shown that the functions of LPCAT1 extend far beyond this, as it also plays important roles in metabolic disorders and tumors—for example, by remodeling the phospholipid composition of cell membranes, it affects membrane fluidity and signal transduction, thereby promoting tumor cell proliferation and migration. Its upregulation has been significantly associated with poor prognosis in tumors such as hepatocellular carcinoma and colorectal cancer, positioning it as an emerging anticancer drug target. Furthermore, in lipid metabolic diseases, LPCAT1 is also involved in regulating inflammatory responses and oxidative stress, playing potential roles in conditions such as atherosclerosis by influencing the generation of oxidized phospholipids.

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Western Blot

WB result of LPCAT1 Recombinant Rabbit mAb
Primary antibody: LPCAT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 μg
Lane 2: A549 whole cell lysate 20 μg
Lane 3: PC-3 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 55 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human lung. Anti-LPCAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human placenta. Anti-LPCAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-LPCAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-LPCAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-LPCAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-LPCAT1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.