WB result of LONP1 Rabbit pAb
Primary antibody: LONP1 Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 106 kDa
LONP1 Rabbit Polyclonal Antibody
LONP1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | LONP1 |
| Synonyms | Lon protease homolog, mitochondrial; LONHs; Lon protease-like protein (LONP); Mitochondrial ATP-dependent protease Lon; Serine protease 15; PRSS15 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P36776 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, HeLa, SH-SY5Y, Jurkat, NIH/3T3, C6 |
| Predicted Reactivity | Bv |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
LONP1 protein, also known as Lon protease homolog 1, is a nuclear-encoded mitochondrial AAA+ serine protease that plays a crucial role in maintaining mitochondrial homeostasis. It is highly conserved across species and is composed of three functional domains: an N-terminal domain for substrate recognition, an AAA+ domain for ATP binding and hydrolysis, and a protease domain for protein degradation. LONP1 forms a hexameric complex in the mitochondrial matrix and is responsible for degrading misfolded, oxidized, and damaged proteins to prevent their accumulation and maintain mitochondrial proteostasis. Additionally, LONP1 has chaperone-like functions in protein folding and assembly, and it regulates mitochondrial DNA (mtDNA) replication and transcription by interacting with mitochondrial transcription factor A (TFAM). Its dysfunction is associated with various diseases, including mitochondrial disorders and cancer, making it a potential target for therapeutic interventions.
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Western Blot
WB result of LONP1 Rabbit pAb
Primary antibody: LONP1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 100 kDa
WB result of LONP1 Rabbit pAb
Primary antibody: LONP1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 100 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-LONP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-LONP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-LONP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-LONP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-LONP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
