LMO4 Recombinant Rat mAb (S-5963)

Catalog Number: S0B60500 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rat
Antigen	LMO4
Synonyms	LIM domain transcription factor LMO4; Breast tumor autoantigen; LIM domain only protein 4 (LMO-4)
Location	Nucleus
Accession	P61968
Clone Number	S-5963
Antibody Type	Rat mAb
Isotype	IgG2a
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, Jurkat, T-47D, NIH/3T3
Purification	Protein G
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms
IHC-P	1:1000	Ms, Rt

Background

LMO4 (LIM Domain Only 4) is a small nuclear protein encoded by the LMO4 gene, belonging to the LIM-only protein family. Its protein structure lacks a DNA-binding domain but instead contains two tandem LIM domains that mediate its interactions with various transcription factors and chromatin remodeling complexes, enabling it to function as a transcriptional adaptor or scaffold molecule involved in regulating gene expression. During physiological development, LMO4 plays critical roles at multiple stages of embryogenesis, particularly being indispensable for mammary epithelial cell proliferation and differentiation, nervous system development (including cerebral cortex formation and synaptic plasticity), and skeletal development. However, aberrant expression of LMO4 is closely associated with various human diseases: it is commonly overexpressed in multiple cancers such as breast cancer, lung cancer, and oral squamous cell carcinoma, where it regulates genes related to cell cycle and epithelial-mesenchymal transition (EMT) through interactions with transcriptional complexes such as core-binding factors (CBF), thereby promoting tumor proliferation and metastasis. Additionally, LMO4 has also been identified as having potential roles in neuropsychiatric disorders through genetic association studies on schizophrenia, Alzheimer's disease, and others, positioning it as a research hotspot at the intersection of cancer and neurological diseases.

Picture

Western Blot

WB result of LMO4 Recombinant Rat mAb
Primary antibody: LMO4 Recombinant Rat mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 μg
Lane 2: Jurkat whole cell lysate 20 μg
Lane 3: T-47D whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rat IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 19 kDa
This blot was developed with high sensitivity substrate

WB result of LMO4 Recombinant Rat mAb
Primary antibody: LMO4 Recombinant Rat mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 μg
Lane 2: Mouse embryo lysate 20 μg
Secondary antibody: Goat Anti-Rat IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 19 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse brain. Anti-LMO4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for rat IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse hippocampus. Anti-LMO4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for rat IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat hippocampus. Anti-LMO4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for rat IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.