LILRB1 Recombinant Rabbit mAb (S-5001)

Catalog Number: S0B60146 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	CD85j
Synonyms	Leukocyte immunoglobulin-like receptor subfamily B member 1; LIR-1; Leukocyte immunoglobulin-like receptor 1; CD85 antigen-like family member J; Immunoglobulin-like transcript 2 (ILT-2); Monocyte/macrophage immunoglobulin-like receptor 7 (MIR-7); ILT2; LIR1; MIR7; LILRB1
Location	Secreted, Cell membrane
Accession	Q8NHL6
Clone Number	S-5001
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	Daudi, Raji
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:2000	Hu

Background

CD85j, also known as LILRB1 or ILT2, is a 110 kDa transmembrane glycoprotein belonging to the leukocyte immunoglobulin-like receptor (LILR) family. It is an inhibitory receptor broadly expressed on leukocytes, including B cells, monocytes, subsets of NK cells, memory/effector CD8+ T cells, gamma δ T cells, and monocyte-derived dendritic cells. CD85j recognizes a wide range of classical HLA-class I allelic variants, as well as non-classical molecules such as HLA-F and HLA-G, by binding to the conserved α3 domain. Additionally, it binds to the human cytomegalovirus-encoded MHC class I homologue UL18. The engagement of CD85j by its ligands induces inhibitory signals that downregulate immune responses, including the inhibition of antigen-induced proliferation and activation of CD8+ T cells, CD4+ T cells, NK cells, and gamma δ T cells. On dendritic cells, ligation of CD85j inhibits the production of cytokines like IL-10, IL-12p70, and TGF-beta and protects from Fas-mediated apoptosis.

Picture

Western Blot

WB result of LILRB1 Recombinant Rabbit mAb
Primary antibody: LILRB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: Daudi whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Negative control: A431 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 110 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-LILRB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-LILRB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-LILRB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-LILRB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-LILRB1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.