Lhx2/LH2 Recombinant Rabbit mAb (S-3867-28)

Catalog Number: S0B6939 Application: WB, IHC-P, IF Reactivity: Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Lhx2/LH2
Synonyms	LIM/homeobox protein Lhx2; Homeobox protein LH-2; LIM homeobox protein 2
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	Q9Z0S2
Clone Number	S-3867-28
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, IF
Reactivity	Ms, Rt
Positive Sample	mouse E14 fetal brain, rat E14 fetal brain
Purification	Protein A
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Ms, Rt
IHC-P	1:4000	Ms, Rt
IF	1:500	Ms

Background

Lhx2, also known as LIM-homeobox 2 or LH2, is a critical transcription factor belonging to the LIM-homeodomain family that plays indispensable roles in embryonic development and tissue maintenance, particularly within the central nervous system, eye, and liver. Characterized by two N-terminal LIM domains that mediate protein-pro interactions and a C-terminal homeodomain responsible for DNA binding, Lhx2 functions primarily as a nuclear regulator of gene expression, often acting in concert with cofactors such as Ldb1 to modulate chromatin accessibility and transcriptional activity. In the developing brain, it is essential for the specification of cortical identity, hippocampal formation, and the differentiation of olfactory sensory neurons, while in the eye, it governs optic cup morphogenesis and retinal progenitor cell proliferation; furthermore, recent studies have highlighted its significance in adult neurogenesis and its potential involvement in pathological conditions, including its tumor-suppressive or oncogenic duality depending on the cellular context, thereby establishing Lhx2 as a multifaceted regulator crucial for both organogenesis and cellular homeostasis.

Picture

Western Blot

WB result of Lhx2/LH2 Recombinant Rabbit mAb
Primary antibody: Lhx2/LH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse E14 fetal brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 50 kDa

WB result of Lhx2/LH2 Recombinant Rabbit mAb
Primary antibody: Lhx2/LH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat E14 fetal brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 50 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse E14 embryo. Anti-Lhx2/LH2 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded nose skin of E14 mouse embryo. Anti-Lhx2/LH2 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat E14 embryo. Anti-Lhx2/LH2 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded olfactory epithelium tissue of E14 rat embryo. Anti-Lhx2/LH2 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunofluorescence

IF shows positive staining in paraffin-embedded E14 mouse embryo. Anti- Lhx2/LH2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.