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LGR5/GPR49 His Tag Protein, Cynomolgus

LGR5/GPR49 His Tag Protein, Cynomolgus

Catalog Number: UA016188 Brand: UA BIOSCIENCE
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$215 USD
$215 USD
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Product Details

Product Specification


Species Human
Synonyms LGR5, GPR49, GPR67
Accession G7PI19
Amino Acid Sequence

Ser22 - Gly557 with His Tag at the C-Terminus

Expression System HEK293
Molecular Weight 70-100kDa (Reducing)
Purity ＞95% by SDS-PAGE
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.
Reference

1. Peng, W.C., et al. (2013). Structure of stem cell growth factor R-spondin 1 in complex with the ectodomain of its receptor LGR5. Cell Reports, 3(6), 1885-1892.
2. Carmon, K.S., et al. (2011). LGR4 and LGR5 are R-spondin receptors mediating Wnt/β-catenin and Wnt/PCP signalling. EMBO Reports, 12(10), 1055-1061.
3. de Lau, W., et al. (2011). LGR5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature, 476(7360), 293-297.

Background

Cynomolgus macaque (Macaca fascicularis) LGR5 is encoded by the LGR5 gene (Gene ID: 102120216) located on chromosome 11. The reference sequence is XM_005571542.4, encoding isoform X1 with protein accession XP_005571599.2. The cynomolgus LGR5 UniProt/TrEMBL accession is A0A2K5WIN4.

Cynomolgus LGR5 is highly homologous to human LGR5 in both structure and function. Recombinant cynomolgus LGR5 protein typically covers the extracellular domain spanning Ser22-Gly557 (corresponding to human LGR5 residues 22-543), with a C-terminal His tag. The protein is expressed in HEK293 cells and exhibits biological activity in binding human R-spondin 1.

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SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

The purity of LGR5 / GPR49 His Tag Protein, Cynomolgus is more than 95% determined by SEC-HPLC.

ELISA

Immobilized Human R-Spondin1/RSPO1 Protein, Human (Cat. No. UA040347) at 5 μg/mL (100 μL/well) can bind LGR5 / GPR49 His Tag Protein, Cynomolgus (Cat. No. UA016188) with a linear range of 8.6-10.06 ng/mL (Routinely tested).