LCK titration in HTRF assay
LCK Protein
LCK Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P06239-1 |
| Amino Acid Sequence | M1-P509(end) |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 83683.0 |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 20mM Tris, 300mM NaCl, 1mM DTT, 10% glycerol, pH7.0 |
| Stability & Storage | -80℃ |
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Bioactivity
The LCK activity was detected using HTRF technology. The reaction was performed by incubating the LCK protein , ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
SEC-HPLC
