WB result of LAMP2 Rabbit pAb
Primary antibody: LAMP2 Rabbit pAb at 1/1000 dilution
Lane 1: JAR whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: SK-MEL-28 whole cell lysate 20 µg
Lane 4: SK-MEL-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 100-130 kDa
LAMP2 Rabbit Polyclonal Antibody
LAMP2 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | LAMP2 |
| Synonyms | Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; LGP-96; CD107b |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P13473 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Mk |
| Positive Sample | JAR, HeLa, SK-MEL-28, SK-MEL-2, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
| ICC | 1:500 | Hu |
Background
CD107b, also known as LAMP2 (Lysosome-Associated Membrane Protein 2), is a heavily glycosylated type I transmembrane protein that constitutes a major component of the lysosomal membrane, where it plays critical roles in maintaining lysosomal integrity, autophagy, and chaperone-mediated autophagy by acting as a receptor for chaperone–substrate complexes on the lysosomal surface.
Picture
Picture
Western Blot
WB result of LAMP2 Rabbit pAb
Primary antibody: LAMP2 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 100-120 kDa
Immunocytochemistry
ICC shows positive staining in JAR cells. Anti- LAMP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
