ICC shows positive staining in HeLa cells. Anti- LAMP1 (FITC Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
LAMP1 Recombinant Rabbit mAb (FITC Conjugate) (SDT-405-39)
LAMP1 Recombinant Rabbit mAb (FITC Conjugate) (SDT-405-39)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | LAMP1 |
| Synonyms | Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; CD107a |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P11279 |
| Clone Number | SDT-405-39 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, ICFCM |
| Reactivity | Hu |
| Positive Sample | HeLa |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:50 | Hu |
| ICFCM | 1:400 | Hu |
Background
Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene. The LAMP-1 glycoprotein is a type I transmembrane protein [PMID: 16973206] which is expressed at high or medium levels in at least 76 different normal tissue cell types. It resides primarily across lysosomal membranes [PMID: 2584229], and functions to provide selectins with carbohydrate ligands. LAMP-1 has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells, and may also play a role in tumor cell differentiation and metastasis.
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Immunocytochemistry
