WB result of Lamin B1 Recombinant Mouse mAb
Primary antibody: Lamin B1 Recombinant Mouse mAb at 1/100000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Lane 6: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 68 kDa
Lamin B1 Recombinant Mouse mAb (S-4865)
Lamin B1 Recombinant Mouse mAb (S-4865)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Lamin B1 |
| Synonyms | Lamin-B1; LMN2; LMNB; LMNB1 |
| Location | Nucleus lamina |
| Accession | P20700 |
| Clone Number | S-4865 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, PC-3, HepG2, HEK-293, Jurkat, K562, NIH/3T3, RAW264.7, 4T1, mouse spleen, PC-12, C6, rat spleen |
| Predicted Reactivity | Rb, CDV, Ck, Zf, Bv, Hm |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:20000-1:100000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:2000 | Hu |
Background
Lamin B1 is a crucial type V intermediate filament protein and a major structural component of the nuclear lamina, a dense fibrillar network located on the inner side of the inner nuclear membrane in eukaryotic cells. Unlike its counterparts Lamin A and C, which are developmentally regulated and often absent in early embryonic stages or certain cell types, Lamin B1 is ubiquitously expressed and considered essential for cell viability, playing indispensable roles in maintaining nuclear shape, mechanical stability, and the spatial organization of chromatin. It serves as a scaffold for anchoring chromatin to the nuclear periphery, thereby influencing gene regulation, DNA replication, and repair processes, while also interacting with various integral membrane proteins to ensure proper nuclear envelope integrity during interphase and facilitating the disassembly and reassembly of the nuclear envelope during mitosis; consequently, dysregulation or mutations in the LMNB1 gene have been linked to severe neurological disorders, such as adult-onset autosomal dominant leukodystrophy, highlighting its critical importance in both cellular architecture and human health.
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Western Blot
WB result of Lamin B1 Recombinant Mouse mAb
Primary antibody: Lamin B1 Recombinant Mouse mAb at 1/100000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: 4T1 whole cell lysate 20 µg
Lane 4: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 68 kDa
WB result of Lamin B1 Recombinant Mouse mAb
Primary antibody: Lamin B1 Recombinant Mouse mAb at 1/100000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 68 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-Lamin B1 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
