WB result of Lamin A/C Recombinant Rabbit mAb
Primary antibody: Lamin A/C Recombinant Rabbit mAb at 1/200000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: A-431 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Lane 6: U-2 OS whole cell lysate 20 µg
Lane 7: Hep G2 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 74 kDa
Observed MW: 74, 64 kDa
Lamin A/C Recombinant Rabbit mAb (S-5671)
Lamin A/C Recombinant Rabbit mAb (S-5671)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Lamin A/C |
| Synonyms | Prelamin-A/C; 70 kDa lamin; Renal carcinoma antigen NY-REN-32; LMN1; LMNA |
| Location | Nucleus |
| Accession | P02545 |
| Clone Number | S-5671 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, S549, A431, PC-3, U-2 OS, HepG2, RAW264.7, NIH/3T3, mouse colon, mouse spleen, mouse liver, C6, PC-12, rat colon, rat spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:20000-1:200000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms, Rt |
Background
Lamin A/C proteins are essential intermediate filament proteins encoded by the LMNA gene through alternative splicing that form a major structural component of the nuclear lamina, a dense fibrillar network underlying the inner nuclear membrane which provides mechanical stability to the nucleus, regulates chromatin organization, and mediates critical cellular processes including DNA replication, transcription, and cell differentiation. These proteins interact with numerous binding partners such as emerin, SUN-domain proteins, and various transcription factors to maintain nuclear architecture and mechanotransduction signaling pathways, making them vital for tissue integrity particularly in mechanically stressed tissues like muscle, fat, and bone. Mutations in the LMNA gene cause a diverse spectrum of disorders collectively termed laminopathies, including Hutchinson-Gilford progeria syndrome, Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and familial partial lipodystrophy, highlighting the protein's multifaceted roles in both structural maintenance and gene regulation, while its post-translational processing—including farnesylation and proteolytic cleavage of prelamin A to mature lamin A—represents a tightly regulated mechanism whose disruption leads to premature aging phenotypes and cellular senescence.
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Western Blot
WB result of Lamin A/C Recombinant Rabbit mAb
Primary antibody: Lamin A/C Recombinant Rabbit mAb at 1/200000 dilution
Lane 1: Raw 264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse colon lysate 20 µg
Lane 4: mouse spleen lysate 20 µg
Lane 5: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 74 kDa
Observed MW: 74, 64 kDa
WB result of Lamin A/C Recombinant Rabbit mAb
Primary antibody: Lamin A/C Recombinant Rabbit mAb at 1/200000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Lane 3: rat colon lysate 20 µg
Lane 4: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 74 kDa
Observed MW: 74, 64 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-Lamin A/C antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Lamin A/C antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Lamin A/C antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Lamin A/C antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Lamin A/C antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Lamin A/C antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Lamin A/C antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells (top panel) and negative staining in Jurkat cells (below panel). Anti-Lamin A/C antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibod at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-Lamin A/C antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in C6 cells. Anti-Lamin A/C antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
