Flow cytometric analysis of Lamin A/C expression on 4% PFA fixed 90% methanol permeabilized HeLa cells. Cells from the HeLa (Human cervix adenocarcinoma epithelial cell) was stained with Alexa Fluor® 488 Rabbit IgG Isotype Control (Black line histogram) and SDT Lamin A/C Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Lamin A/C Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-R041)
Lamin A/C Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-R041)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Lamin A/C |
| Synonyms | Prelamin-A/C; 70 kDa lamin; Renal carcinoma antigen NY-REN-32 |
| Location | Nucleus lamina |
| Accession | P02545 |
| Clone Number | SDT-R041 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, ICFCM |
| Reactivity | Hu |
| Positive Sample | HeLa |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:200 | Hu |
| ICFCM | 1:800 | Hu |
Background
Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. Lamin A has a mass of about 74kDa while Lamin C is 65kDa. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Lamin A/C (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
