Use the L separation column to enrich or deplete CD3+ T cells from Jurkat (Human T cell leukemia T lymphocyte, Right) or HL-60 (Human acute promyelocytic leukemia cell line, Left). CD3+ cells were stained with anti-CD3 (clone: UCHT1) Alexa Fluor™ 647 and gated on Live+.
L Separation Columns
L Separation Columns
Price:
Regular price
$420 USD
Regular price
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$420 USD
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Product Details
Product Details
Product Specification
| Capacity | 1x108 magnetically labeled cells in 2x109 total cells.Recommended sample size for leukocytes: 105 - 108 labeled cells in 107 - 2x109 total cells.Note: Column capacity may decrease when separating cells larger than lymphocytes. |
| Separation Method | Column-Based |
| Stability & Storage | Store columns dry at +10 to +35 °C. |
Background
The column Technology is based on the use of NanoBeads, Columns, and Separators. Separation have been developed for the gentle isolation of NanoBead-labeled cells. As NanoBeads are extremely small, superparamagnetic particles, a high-gradient magnetic field is required to retain the labeled cells. Separation contains an optimized matrix to generate this strong magnetic field when placed in a permanent magnet, such as the Starter MagSep Separator (L) or Starter MagSep Separator (4L).
Components
25 L Separation Columns and plungers, sterile packed
Picture
Picture
Validation Data
Beads: CD8 Nanobeads, mouse(S0K0005)
Column: L separation column(S0D3011)
