WB result of S-RMabMix™ L-Lactyl Lysine Rabbit mAb
Primary antibody: S-RMabMix™ L-Lactyl Lysine Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
S-RMabMixTM L-Lactyl Lysine Rabbit mAb
S-RMabMixTM L-Lactyl Lysine Rabbit mAb
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$220 USD
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$220 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | L-Kla, Kla, L‑乳酸化，L-乳酰化 |
| Antibody Type | Recombinant mAb Mix |
| Isotype | IgG |
| Application | WB, ICC, IP |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | |
| IP | 1:50 | |
| ICC | 1:500 |
Background
Lysine lactylation is an emerging post-translational modification that occurs in cellular metabolism, specifically referring to the accumulation of lactic acid modifying lysine residues on histones. Lysine lactylation modifies the chromatin structure, thereby affecting gene expression. Research has found that lactylation sites are widely distributed in gene promoter regions, closely associated with gene transcriptional activation. Lysine lactylation also participates in energy metabolism processes, potentially related to the dynamic balance regulation of histone lysine acetylation.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of S-RMabMix™ L-Lactyl Lysine Rabbit mAb
Lane 1: Modified peptide library (L-lactyl lysine)
Lane 2: Unmodified peptide library control
Lane 3: Modified peptide library (D-lactyl lysine)
Lane 4: Modified peptide library (β-hydroxybutyryl lysine)
Lane 5: Modified peptide library (Fumaryl lysine)
Lane 6: Modified peptide library (Crotonyl lysine)
Lane 7: Modified peptide library (Succinyl lysine)
Lane 8: Modified peptide library (Butyryl lysine)
Lane 9: Modified peptide library (Propionyl lysine)
Lane 10: Modified peptide library (Actyl lysine)
Primary antibody: S-RMabMix™ L-Lactyl Lysine Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
