KRAS-G12C activity test using HTRF method
The KRAS-G12C activity was assayed with HTRF technology. The reaction was performed by incubating the KRAS-G12C protein, GTP, cRAF and beads at 25℃ for 60 min, then reading Ratio 665/620nm signal with BMG.
KRAS [G12C] His Tag Protein
KRAS [G12C] His Tag Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | C-K-RAS, CFC2, K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RALD, RASK2 |
| Accession | P01116-2 |
| Amino Acid Sequence | M1-K169 |
| Expression System | E.coli |
| Molecular Weight | 21690.0 |
| Purity | >90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.4 ,10% glycerol |
| Stability & Storage | -80℃ |
Picture
Picture
ELISA
Molecular Interaction
KRAS [G12C] titration
