KRAS-G12C activity test using HTRF method
The KRAS-G12C activity was assayed with HTRF technology. The reaction was performed by incubating the KRAS-G12C protein, GTP, cRAF and beads at 25℃ for 60 min, then reading Ratio 665/620nm signal with BMG.
KRAS [G12C] His Tag Protein, Human(Load GDP/GMPPNP)
KRAS [G12C] His Tag Protein, Human(Load GDP/GMPPNP)
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | C-K-RAS,CFC2,K-RAS,K-RAS2A,K-RAS2B,K-RAS4A,K-RAS4B,KI-RAS,KRAS1,KRAS2,NS,NS3,RALD,RASK2 |
| Accession | P01116-2 |
| Amino Acid Sequence | M1-K169 |
| Expression System | E.coli |
| Molecular Weight | 21690.0 |
| Purity | >90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.4 ,10% glycerol |
| Stability & Storage | -80℃ |
Picture
Picture
ELISA
Molecular Interaction
KRAS [G12C] titration
![KRAS [G12C] His Tag Protein, Human(Load GDP/GMPPNP)](http://www.antbioinc.com/cdn/shop/files/0ac35e05-b496-4707-aa43-ac4d93806cdf.png?v=1787652258&width=1445)
![KRAS [G12C] His Tag Protein, Human(Load GDP/GMPPNP)](http://www.antbioinc.com/cdn/shop/files/414974a4-2789-47d6-96eb-d3c09aec9840.png?v=1787652258&width=1445)