KRAS-WT titration
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | C-K-RAS, CFC2, K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RALD, RASK2 |
| Accession | P01116-2 |
| Amino Acid Sequence | M1-K169 |
| Expression System | E.coli |
| Molecular Weight | 23542.0 |
| Purity | >90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris ,300 mM NaCl , 1 mM DTT, pH7.5 ,10% glycerol |
| Stability & Storage | -80℃ |
Picture
Picture
Molecular Interaction


KRAS-WT activity test using HTRF method
The KRAS-WT activity was assayed with HTRF technology. The reaction was performed by incubating the KRAS- WT protein, GTP, cRAF and beads at 25℃ for 60 min, then reading Ratio 665/620nm signal with BMG.
