1 2

KLRG1 Fc Chimera Protein, Human

KLRG1 Fc Chimera Protein, Human

Catalog Number: UA016150 Brand: UA BIOSCIENCE
Share:
Price:
$215 USD
$215 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Species Human
Synonyms KLRG1, CLEC15A, MAFA, MAFAL
Accession Q96E93
Amino Acid Sequence

Leu 60 - Phe 195 with hIgG1 Fc Tag at the C Terminus

Expression System HEK293
Molecular Weight 55-70kDa (Reducing)
Purity ＞95% by SDS-PAGE
Conjugation Unconjugated
Tag Human IgG1 Fc
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.
Reference

1.Li, Y., et al. (2009). Structure of natural killer cell receptor KLRG1 bound to E-cadherin reveals basis for MHC-independent missing self recognition. Immunity, 31(1), 35-46.
2.Ito, M., et al. (2006). Killer cell lectin-like receptor G1 binds three members of the classical cadherin family to inhibit NK cell cytotoxicity. Journal of Experimental Medicine, 203(2), 289-295.
3. Henson, S. M., & Akbar, A. N. (2009). KLRG1—more than a marker for T cell senescence. Age, 31(4), 285-291.

Background

KLRG1 (Killer Cell Lectin-Like Receptor G1) is an inhibitory immune checkpoint receptor belonging to the C-type lectin-like receptor family. It is encoded by the KLRG1 gene located on human chromosome 12 and is also known by the aliases MAFA, 2F1, MAFA-L, and CLEC15A. The human KLRG1 protein consists of 195 amino acids and is a type II transmembrane glycoprotein with a molecular weight of approximately 30 kDa. Structurally, KLRG1 contains an extracellular C-type lectin-like domain (CTLD) for ligand recognition and an intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM) for signal transduction.

KLRG1 is predominantly expressed on NK cells and subsets of effector and memory T cells. Its classical ligands are members of the classical cadherin family, including E-cadherin, N-cadherin, and R-cadherin. KLRG1 recognizes and binds to a highly conserved Val3-Ile7 sequence within the N-terminal EC1 domain of these cadherins via its CTLD.

Picture

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

ELISA

Immobilized KLRG1 Fc Chimera Protein, Human (Cat. No. UA016150) at 1 μg/mL (100 μL/well) can bind Anti-Human KLRG1 Monoclonal Antibody (Ulviprubart) (Cat. No. UA016212) with EC50 of 1.40-2.12 ng/mL.