KLK5 Recombinant Rabbit mAb (S-4238-55)

Catalog Number: S0B60184 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	KLK5
Synonyms	Kallikrein-5; Kallikrein-like protein 2 (KLK-L2); Stratum corneum tryptic enzyme; SCTE
Immunogen	Synthetic Peptide
Location	Secreted
Accession	Q9Y337
Clone Number	S-4238-55
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	Mouse skin, rat skin, rat stomach
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:500-1:1000	Ms, Rt
IHC-P	1:500	Hu, Ms, Rt

Background

KLK5 also known as stratum corneum tryptic enzyme (SCTE), is a secreted serine protease encoded by the KLK5 gene. This protein is initially synthesized as a zymogen containing a signal peptide and an activation peptide, and after proteolytic processing, it forms a mature enzyme composed of 227 amino acid residues, exhibiting typical trypsin-like activity with a preference for cleavage after arginine (Arg) residues. Under physiological conditions, KLK5 plays a central role in skin desquamation by degrading cell adhesion molecules in the stratum corneum to promote the shedding of dead cells; simultaneously, it can activate other kallikreins such as KLK7 and KLK14, forming a proteolytic cascade. In terms of pathology, dysregulation of KLK5 function is associated with various diseases: in skin disorders, hyperactivation of KLK5 is a key driver of Netherton syndrome (a severe hereditary skin disease), leading to skin barrier defects and severe inflammation; in the field of oncology, KLK5 shows aberrant expression in various cancers including ovarian cancer and breast cancer, though its exact mechanism of action remains under investigation; additionally, recent studies have found that KLK5 can be upregulated upon beta-coronavirus infection and directly cleave the viral spike protein to promote viral replication, making it a promising drug target against coronaviruses.

Picture

Western Blot

WB result of KLK5 Recombinant Rabbit mAb
Primary antibody: KLK5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skin lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 30-38 kDa

WB result of KLK5 Recombinant Rabbit mAb
Primary antibody: KLK5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skin lysate 20 µg
Lane 2: rat stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 27-37 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human skin. Anti-KLK5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse skin. Anti-KLK5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat skin. Anti-KLK5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.