KLHL21 Recombinant Rabbit mAb (S-3460-252)

Catalog Number: S0B6938 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	KLHL21
Synonyms	Kelch-like protein 21; KIAA0469
Immunogen	Synthetic Peptide
Location	Cytoplasm, Cytoskeleton
Accession	Q9UJP4
Clone Number	S-3460-252
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	A431, HeLa, A549, U-87 MG, BxPC-3, Raji, HepG2, MCF7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20°C as supplied

Dilution

application	dilution	species
WB	1:500-1:1000	Hu
IHC-P	1:500	Hu

Background

KLHL21 (Kelch-like family member 21) is a substrate-specific adaptor protein for the Cullin 3-based E3 ubiquitin ligase complex, playing a pivotal role in regulating cellular processes through targeted protein ubiquitination and subsequent degradation. Characterized by its distinctive domain architecture, which includes an N-terminal BTB/POZ domain responsible for binding to Cullin 3 and a C-terminal kelch repeat domain that mediates specific substrate recognition, KLHL21 functions as a critical bridge between the ubiquitination machinery and its target proteins. It is particularly renowned for its essential function in mitosis, where it localizes to chromosomes and the central spindle to regulate the Aurora B kinase pathway, thereby ensuring accurate chromosome segregation and cytokinesis; dysregulation or mutation of KLHL21 has been implicated in various pathological conditions, including neurodevelopmental disorders and certain cancers, highlighting its significance in maintaining genomic stability and proper cell division.

Picture

Western Blot

WB result of KLHL21 Recombinant Rabbit mAb
Primary antibody: KLHL21 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Lane 5: BxPC-3 whole cell lysate 20 µg
Lane 6: Raji whole cell lysate 20 µg
Lane 7: HepG2 whole cell lysate 20 µg
Lane 8: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 67 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-KLHL21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-KLHL21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreas cancer. Anti-KLHL21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.