KLC1 Recombinant Rabbit mAb (S-4556-8)

Catalog Number: S0B60671 Application: WB, IHC-P, ICC Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	KLC1
Synonyms	Kinesin light chain 1; KLC; KNS2
Immunogen	Synthetic Peptide
Location	Cytoplasm, Cytoskeleton
Accession	Q07866
Clone Number	S-4556-8
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms, Rt
Positive Sample	293T, HeLa, U-87 MG, Neuro-2a, C2C12, mouse brain, PC-12, rat brain, COS-7
Predicted Reactivity	Or
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:1000	Hu, Ms, Rt
ICC	1:500	Hu, Ms

Background

KLC1 (kinesin light chain 1) is the light chain subunit of conventional kinesin-1, encoded by the KLC1 gene, and is predominantly expressed in neuronal tissues. Kinesin-1 is a heterotetramer composed of two heavy chains (KIF5A/B/C) and two light chains, in which the heavy chains generate the force for movement along microtubules toward the plus end, while the light chains themselves are not motor proteins but primarily serve as adaptor molecules. They bind to the heavy chains through their N-terminal domain and interact with a variety of cargoes—including vesicles, mitochondria, and the Golgi apparatus—via six tetratricopeptide repeat (TPR) motifs. The function of KLC1 is particularly critical for neurons: KLC1 knockout mice are significantly smaller in size and exhibit pronounced motor impairments, with KIF5A abnormally co-localizing with cis-Golgi markers in their sensory and motor neurons, and the intracellular distribution of KIF5B and β'-COP also being markedly altered. These findings indicate that KLC1 is essential for the proper activation or targeting of kinesin heavy chains. Furthermore, KLC1 also interacts with specific cargo receptors such as the mitochondrial outer membrane protein FUNDC1 through its TPR domain, further expanding its adaptor functions in intracellular material transport.

Picture

Western Blot

WB result of KLC1 Recombinant Rabbit mAb
Primary antibody: KLC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65, 75 kDa

WB result of KLC1 Recombinant Rabbit mAb
Primary antibody: KLC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65, 75 kDa

WB result of KLC1 Recombinant Rabbit mAb
Primary antibody: KLC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65, 75 kDa

WB result of KLC1 Recombinant Rabbit mAb
Primary antibody: KLC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65, 75 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human brain. Anti-KLC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-KLC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse brain. Anti-KLC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat brain. Anti-KLC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HeLa cells. Anti- KLC1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

ICC shows positive staining in Neuro-2a cells. Anti- KLC1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).