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KLC1 Recombinant Rabbit mAb (S-4556-17)

KLC1 Recombinant Rabbit mAb (S-4556-17)

Catalog Number: S0B60276 Application: WB, IHC-P Reactivity: Hu, Mk Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen KLC1
Synonyms Kinesin light chain 1; KLC; KNS2; KLC1
Immunogen Synthetic Peptide
Location Cytoplasm, Cytoskeleton
Accession Q07866
Clone Number S-4556-17
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu, Mk
Positive Sample 293T, HeLa, U-87 MG, HT-29, COS7
Purification Protein A
Concentration 2 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu, Mk
IHC-P 1:500-1:1000 Hu

Background

KLC1 (Kinesin Light Chain 1) is a component of the kinesin-1 motor protein complex that plays a crucial role in microtubule-based intracellular transport. It functions primarily by coupling specific cargo (such as organelles and vesicles) to the kinesin heavy chain and modulating its ATPase activity. Highly abundant in the brain and spine, KLC1 is essential for axo-dendritic transport, and mutations in the KLC1 gene have been linked to neurodevelopmental disorders and movement abnormalities like dystonia.

Picture

Western Blot

WB result of KLC1 Recombinant Rabbit mAb
Primary antibody: KLC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65-75 kDa


WB result of KLC1 Recombinant Rabbit mAb
Primary antibody: KLC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65-75 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-KLC1 antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-KLC1 antibody was used at 1/800 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.