Flow cytometric analysis of KIM-1 expression on A549(Human lung carcinoma epithelial cell). Cells from the A549 (right panel) or Hela (Human cervix adenocarcinoma epithelial cell, left panel) was stained with Alexa Fluor® 647 Rabbit IgG Isotype Control (black line histogram) or SDT KIM-1 Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (red line histogram) at 1:1000 dilution (0.1 μg). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | KIM-1 |
| Synonyms | Hepatitis A virus cellular receptor 1; Kidney injury molecule 1 (KIM-1); T-cell immunoglobulin and mucin domain-containing protein 1 (TIMD-1); T-cell immunoglobulin mucin receptor 1 (TIM; TIM-1); T-cell membrane protein 1; CD365; KIM1; TIM1; TIMD1; HAVCR1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q96D42 |
| Clone Number | S-932-2 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | A549 |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:1000 | Hu |
Background
KIM-1 is a protein the most highly upregulated in injured kidneys by various types of insults. Its upregulation during renal injury has been found in the kidneys of the vertebrates such as Zebrafish and humans. KIM-1 is also a member of the TIM (T cell transmembrane, immunoglobulin, and mucin) gene family, which plays critical roles in regulating immune cell activity especially regarding the host response to viral infection. It is involved in allergic response, asthma, and transplant tolerance.
Picture
Picture
FC
