WB result of KIF5B+KIF5C Recombinant Rabbit mAb
Primary antibody: KIF5B+KIF5C Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: HCT 116 whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 110 kDa
Observed MW: 115 kDa
KIF5B+KIF5C Recombinant Rabbit mAb (S-2452-15)
KIF5B+KIF5C Recombinant Rabbit mAb (S-2452-15)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | KIF5B |
| Synonyms | Kinesin-1 heavy chain; Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain (UKHC); KNS; KNS1; Kinesin heavy chain isoform 5C; Kinesin heavy chain neuron-specific 2; Kinesin-1; KIF5C; KIAA0531; NKHC2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P33176、 O60282 |
| Clone Number | S-2452-15 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat, MCF7, HCT 116, HepG2, Neuro-2a, C2C12, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
| ICC | 1:100 | Hu |
| ICFCM | 1:50 | Hu |
Background
KIF5B and KIF5C are two major members of the Kinesin-1 family of motor proteins, encoded by distinct genes. They share a similar core structure, each containing a conserved N-terminal motor domain (which binds to microtubules and hydrolyzes ATP to generate motive force), a neck linker region, a coiled-coil stalk region (mediating dimerization), and a C-terminal tail domain (responsible for binding to various cargoes and regulation). However, they exhibit key differences in tissue distribution and function. KIF5B is widely expressed in nearly all cell types and is responsible for fundamental "housekeeping" transport processes, such as the movement of organelles like mitochondria, endoplasmic reticulum, and endosomes along microtubules. In contrast, KIF5C is predominantly and highly expressed in neurons, making it a neuron-specific kinesin critical for neuronal development and function. It is essential for the anterograde transport of specific cargoes, including presynaptic components, vesicles, and mRNAs, from the neuronal cell body along the axon to distal synapses. This transport is indispensable for maintaining neuronal polarity and synaptic plasticity. The differences in their tail domains determine their ability to recognize and bind distinct cargo adaptor proteins, enabling fine-tuned functional specialization in intracellular transport.
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Western Blot
WB result of KIF5B+KIF5C Recombinant Rabbit mAb
Primary antibody: KIF5B+KIF5C Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 110 kDa
Observed MW: 115 kDa
WB result of KIF5B+KIF5C Recombinant Rabbit mAb
Primary antibody: KIF5B+KIF5C Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 110 kDa
Observed MW: 120 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labelling KIF5B+KIF5C antibody at 1/50 dilution (1 μg)/ (red) compared with a Rabbit monoclonal IgG (black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-KIF5B+KIF5C antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-KIF5B+KIF5C antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
