WB result of KIF18A Recombinant Rabbit mAb
Primary antibody: KIF18A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDa
KIF18A Recombinant Rabbit mAb (S-3310-11)
KIF18A Recombinant Rabbit mAb (S-3310-11)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | KIF18A |
| Synonyms | Kinesin-like protein KIF18A; Marrow stromal KIF18A (MS-KIF18A) |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton, Nucleus |
| Accession | Q8NI77 |
| Clone Number | S-3310-11 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, A549, Jurkat, MCF7, F9, Neuro-2a, raw264.7, rat testis, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu |
Background
KIF18A is a kinesin-8 family motor protein that uses ATP hydrolysis to move along microtubules, selectively accumulating at the plus ends of kinetochore fibers where it dampens their dynamics to ensure precise chromosome congression on the metaphase plate, control spindle length and tension, and coordinate subsequent cytokinesis, thereby safeguarding genomic integrity; its depletion triggers metaphase alignment errors, anaphase delay, central-spindle disorganization, and incomplete cleavage furrow formation, while its overexpression is strongly linked to aggressive cancers with chromosomal instability, making it a promising selective therapeutic target for small-molecule inhibitors that induce mitotic catastrophe preferentially in aneuploid tumor cells.
Picture
Picture
Western Blot
WB result of KIF18A Recombinant Rabbit mAb
Primary antibody: KIF18A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: F9 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Lane 3: raw264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDa
WB result of KIF18A Recombinant Rabbit mAb
Primary antibody: KIF18A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDa
WB result of KIF18A Recombinant Rabbit mAb
Primary antibody: KIF18A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-KIF18A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-KIF18A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-KIF18A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
