IHC shows positive staining in paraffin-embedded mouse colon. Anti-Ki67 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Ki67 Recombinant Rabbit mAb (S-4923-15)
Ki67 Recombinant Rabbit mAb (S-4923-15)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Ki67 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | E9PVX6 |
| Clone Number | S-4923-15 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:2000 | Ms, Rt |
| IF | 1:100-1:500 | Ms, Rt |
Background
Ki67 is a non-histone nuclear protein encoded by the MKI67 gene, widely used as a proliferation marker in pathology. It is expressed during all active phases of the cell cycle—G1, S, G2, and M—but is absent in quiescent (G0) cells. Ki67 has multiple molecular functions, including forming the perichromosomal layer to prevent chromosome aggregation during mitosis and organizing heterochromatin. It also facilitates chromosome attachment to the mitotic spindle and individual chromosome mobility. In addition to its role in cell division, Ki67 is a valuable prognostic and predictive indicator in cancer diagnosis, with its expression levels correlating with tumor cell proliferation rates.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Ki67 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-Ki67 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse colon. Anti-Ki67 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse spleen. Anti-Ki67 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat spleen. Anti-Ki67 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
