1μg (R: reducing condition, N: non-reducing condition).
KEAP1 GST&His Tag Protein, Human
KEAP1 GST&His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | Q14145 |
| Amino Acid Sequence | Met1-Cys624, with C-terminal His |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 94kDa (Reducing) |
| Purity | ＞85% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
Mol Cell Biol.2005 Jan;25(1):162-71. |
Background
KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1)complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2. The BCR(KEAP1)complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation .It also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome.
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