KDM2A Recombinant Rabbit mAb (S-3943-25)

Catalog Number: S0B6907 Application: WB, IHC-P Reactivity: Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25µl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	KDM2A
Synonyms	Lysine-specific demethylase 2A; F-box and leucine-rich repeat protein 11; F-box/LRR-repeat protein 11; JmjC domain-containing histone demethylation protein 1A; [Histone-H3]-lysine-36 demethylase 1A; Fbl11; Fbxl11; Jhdm1a; Kiaa1004; Kdm2a
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	P59997
Clone Number	S-3943-25
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Ms, Rt
Positive Sample	NIH/3T3, RAW264.7, mouse testis, PC-12, C6
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:500-1:1000	Ms, Rt
IHC-P	1:100	Ms, Rt

Background

KDM2A (also known as FBXL11 or JHDM1A) is a multifunctional protein that primarily functions as a histone H3 lysine 36 (H3K36) demethylase through its JmjC domain, specifically removing H3K36me1/2 marks. As a key epigenetic regulator, it binds specifically to unmethylated CpG islands in the genome via its CXXC zinc finger domain, thereby influencing chromatin structure and gene expression. Additionally, KDM2A contains an F-box domain that enables it to act as a substrate recognition subunit of the SCF (SKP1-Cullin-F-box) E3 ubiquitin ligase complex, participating in protein ubiquitination and degradation. Through its enzymatic activity and protein interactions, KDM2A is widely involved in regulating critical biological processes such as cell proliferation, differentiation, DNA damage repair, and maintenance of genomic stability. It also plays complex, and sometimes contradictory, regulatory roles in various cancers, including non-small cell lung cancer and breast cancer.

Picture

Western Blot

WB result of KDM2A Recombinant Rabbit mAb
Primary antibody: KDM2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 130 kDa
This blot was developed with high sensitivity substrate

WB result of KDM2A Recombinant Rabbit mAb
Primary antibody: KDM2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 130 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse brain. Anti-KDM2A antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti-KDM2A antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat brain. Anti-KDM2A antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.