WB result of KDM1B Recombinant Rabbit mAb
Primary antibody: KDM1B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Weak expression: HepG2 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 92 kDa
This blot was developed with high sensitivity substrate
KDM1B Recombinant Rabbit mAb (S-993-8)
KDM1B Recombinant Rabbit mAb (S-993-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Lysine-specific histone demethylase 2, Flavin-containing amine oxidase domain-containing protein 1, Lysine-specific histone demethylase 1B, AOF1, C6orf193, LSD2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q8NB78 |
| Clone Number | S-993-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| ICC | 1:400-1:2000 | |
| ICFCM | 1:200 |
Background
KDM1B, also known as LSD2 or Aof1, is an enzyme that plays crucial roles in various biological processes. It has FAD-binding activity and FAD-dependent demethylase activity specific to histone H3-di/monomethyl-lysine-4. It is involved in the regulation of DNA methylation, particularly upstream of spermatogenesis, histone H3-K4 demethylation, and the regulation of gene expression by genetic imprinting.
Picture
Picture
Western Blot
WB result of KDM1B Recombinant Rabbit mAb
Primary antibody: KDM1B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 92 kDa
WB result of KDM1B Recombinant Rabbit mAb
Primary antibody: KDM1B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 92 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling KDM1B antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-KDM1B antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
