KCNMA1 Recombinant Rabbit mAb (S-2926-33)

Catalog Number: S0B60144 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	KCNMA1
Synonyms	Calcium-activated potassium channel subunit alpha-1; BK channel; BKCA alpha; Calcium-activated potassium channel; subfamily M subunit alpha-1; K(VCA)alpha; KCa1.1; KCNMA; SLO
Immunogen	Synthetic Peptide
Location	Cell membrane
Accession	Q12791
Clone Number	S-2926-33
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	SH-SY5Y, Neuro-2a
Predicted Reactivity	Xe, Zf, Rb, Bv, Dg, Pg, Mq, Ck
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms
IHC-P	1:2000	Hu, Ms, Rt

Background

The α subunit encoded by the KCNMA1 gene is the core pore-forming protein that constitutes the large-conductance calcium-activated potassium channel (BK channel, also known as MaxiK or KCa1.1). This protein has a molecular weight of approximately 134 kDa and belongs to the voltage-gated potassium channel superfamily. Its unique feature lies in its ability to respond simultaneously to membrane depolarization and increases in intracellular calcium concentration, mediating potassium efflux to rapidly repolarize or hyperpolarize the cell membrane, thereby precisely regulating the excitability of various excitable cells, including neurons and smooth muscle cells. Structurally, four identical subunits of the KCNMA1 protein assemble into a homotetramer, with each subunit containing seven transmembrane segments (S0–S6) and a large intracellular C-terminal domain. The S4 segment serves as the voltage sensor, while the C-terminus contains two RCK domains harboring high-affinity calcium-binding sites known as the "calcium bowl" as well as magnesium-binding sites, both of which cooperatively mediate calcium/magnesium-dependent channel activation. Through this unique dual activation mechanism, KCNMA1 plays a central role in regulating vascular smooth muscle tone, controlling neurotransmitter release, maintaining auditory tuning in cochlear hair cells, and participating in innate immunity, among other critical physiological processes. Furthermore, the function of this protein is finely modulated by alternative splicing, phosphorylation, and other post-translational modifications, as well as by association with auxiliary β or γ subunits. Both loss-of-function and gain-of-function mutations in the KCNMA1 gene are closely associated with a spectrum of neurological disorders, including generalized epilepsy, paroxysmal dyskinesia, and ataxia.

Picture

Western Blot

WB result of KCNMA1 Recombinant Rabbit mAb
Primary antibody: KCNMA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 137 kDa
Observed MW: 150 kDa

WB result of KCNMA1 Recombinant Rabbit mAb
Primary antibody: KCNMA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 137 kDa
Observed MW: 150 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-KCNMA1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.