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KCND2 Recombinant Rabbit mAb (S-3102-32)

KCND2 Recombinant Rabbit mAb (S-3102-32)

Catalog Number: S0B6543 Application: WB, IHC-P Reactivity: Mouse, Rat Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen KCND2
Synonyms A-type voltage-gated potassium channel KCND2; Potassium voltage-gated channel subfamily D member 2; Voltage-gated potassium channel subunit Kv4.2; KIAA1044
Immunogen Synthetic Peptide
Location Cell membrane, Cell projection
Accession Q9NZV8
Clone Number S-3102-32
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Ms, Rt
Positive Sample unboiled mouse brain, unboiled mouse cerebellum, unboiled rat brain, unboiled rat cerebellum
Predicted Reactivity Rb, Ft
Purification Protein A
Concentration 2 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Ms, Rt
IHC-P 1:500 Ms, Rt

Background

KCND2, also known as Kv4.2, is a voltage-gated, Shal-type potassium channel subunit that generates rapidly inactivating A-type outward K⁺ currents essential for the early repolarization phase (phase 1) of cardiac and neuronal action potentials; it is expressed most abundantly in brain and heart, forms tetrameric channels at the plasma membrane (including dendritic spines and T-tubules) where it interacts with auxiliary subunits and the actin-binding protein FLNC to fine-tune excitability, and its gain- or loss-of-function has been linked to cardiac arrhythmias, epilepsy and developmental neurological disorders.

Picture

Western Blot

WB result of KCND2 Recombinant Rabbit mAb
Primary antibody: KCND2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse liver lysate 20 µg
Lane 2: unboiled mouse brain lysate 20 µg
Lane 3: unboiled mouse cerebellum lysate 20 µg
Negative control: unboiled mouse liver lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 250, 300, 70 kDa

WB result of KCND2 Recombinant Rabbit mAb
Primary antibody: KCND2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat liver lysate 20 µg
Lane 2: unboiled rat brain lysate 20 µg
Lane 3: unboiled rat cerebellum lysate 20 µg
Negative control: unboiled rat liver lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 250, 300, 70 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-KCND2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-KCND2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-KCND2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-KCND2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat liver. Anti-KCND2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.