WB result of KCC2 Recombinant Rabbit mAb
Primary antibody: KCC2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 126 kDa
Observed MW: 180 kDa
KCC2 Recombinant Rabbit mAb (S-3044-2)
KCC2 Recombinant Rabbit mAb (S-3044-2)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | KCC2 |
| Synonyms | Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2 (hKCC2); Neuronal K-Cl cotransporter; KIAA1176; SLC12A5 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q9H2X9 |
| Clone Number | S-3044-2 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | unboiled mouse brain, unboiled rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:500 | Ms, Rt |
Background
KCC2 (potassium-chloride cotransporter 2) is a neuron-specific chloride-extruding transporter encoded by the SLC12A5 gene that maintains low intracellular Cl⁻ by coupling K⁺ and Cl⁻ efflux, thereby setting the hyperpolarizing, inhibitory polarity of GABA_A and glycine receptor currents essential for central nervous system inhibition, synapse maturation, dendritic spine formation, and neuronal survival, while its transcriptional and post-translational regulation (phosphorylation, membrane trafficking, activity-dependent expression) drives the developmental GABA “switch” from depolarizing to hyperpolarizing, and its down-regulation or dysfunction—triggered by epilepsy, trauma, inflammation, or genetic mutations—produces chloride accumulation, excitatory GABA, network hyperexcitability, and contributes to epilepsies, chronic pain, autism, and neurodegeneration, making KCC2 a genetically validated therapeutic target whose potentiation can restore inhibition and rescue drug-resistant neurological disorders.
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Picture
Western Blot
WB result of KCC2 Recombinant Rabbit mAb
Primary antibody: KCC2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 126 kDa
Observed MW: 180 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-KCC2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-KCC2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-KCC2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-KCC2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebral cortex. Anti-KCC2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
