WB result of Jak2 Recombinant Rabbit mAb
Primary antibody: Jak2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: TF-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 131 kDa
Observed MW: 120 kDa
Jak2 Recombinant Rabbit mAb (S-1316-5)
Jak2 Recombinant Rabbit mAb (S-1316-5)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Jak2 |
| Synonyms | Tyrosine-protein kinase JAK2, Janus kinase 2 (JAK-2) |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | O60674 |
| Clone Number | S-1316-5 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| ICC | 1:100-1:500 | null |
| ICFCM | 1:500 | null |
Background
Jak2, also known as Janus Kinase 2, is a non-receptor tyrosine kinase enzyme that plays a crucial role in cellular signaling pathways. It belongs to the Janus kinase (JAK) family of enzymes, which are involved in the transmission of signals from cell surface receptors to the nucleus. Jak2 is activated by various cytokines, including erythropoietin (EPO), thrombopoietin (TPO), and growth hormone (GH), among others. Upon activation, Jak2 phosphorylates specific tyrosine residues on the receptor and on downstream signaling molecules, initiating a cascade of cellular responses. Jak2 is essential for normal hematopoiesis, as it regulates the proliferation, differentiation, and survival of hematopoietic stem cells and progenitor cells. Mutations in Jak2 can lead to hematological disorders, such as polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF). Jak2 also plays a role in immune responses by modulating the activity of immune cells, including T cells, B cells, and natural killer (NK) cells. It contributes to the regulation of cytokine production and the activation of immune effector functions.
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Western Blot
WB result of Jak2 Recombinant Rabbit mAb
Primary antibody: Jak2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 131 kDa
Observed MW: 120 kDa
WB result of Jak2 Recombinant Rabbit mAb
Primary antibody: Jak2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 131 kDa
Observed MW: 120 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized K562 (Human chronic myelogenous leukemia lymphoblast) labelling Jak2 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC shows positive staining in K562 cells. Anti-Jak2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-Jak2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
