WB result of Jak1 Recombinant Rabbit mAb
Primary antibody: Jak1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: 22RV1 whole cell lysate 20 µg
Lane 3: HDLM-2 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Lane 6: DU 145 whole cell lysate 20 µg
Low expression control: LNCaP whole cell lysate; 22RV1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 120 kDa
Jak1 Recombinant Rabbit mAb (S-4201)
Jak1 Recombinant Rabbit mAb (S-4201)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | JAK1 |
| Synonyms | Tyrosine-protein kinase JAK1; Janus kinase 1 (JAK-1); JAK1A; JAK1B; JAK1 |
| Accession | P23458 |
| Clone Number | S-4201 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HDLM-2, K562, PC-3, DU 145, CTLL-2, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
Jak1 (Janus kinase 1) is a 130-kDa, membrane-proximal tyrosine kinase that associates with the cytoplasmic tails of cytokine receptors—such as those for interferons, interleukins and gp130 family ligands—and, upon ligand-driven receptor clustering, trans-autophosphorylates its activation loop and then phosphorylates STAT proteins to trigger rapid gene-expression programs governing immunity, hematopoiesis and growth; because its ATP-binding cleft can be occupied by ruxolitinib, upadacitinib or other clinical inhibitors, Jak1 is a validated drug target in autoimmune diseases, myeloproliferative neoplasms and alloimmune disorders.
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Picture
Western Blot
WB result of Jak1 Recombinant Rabbit mAb
Primary antibody: Jak1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: CTLL-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 120 kDa
WB result of Jak1 Recombinant Rabbit mAb
Primary antibody: Jak1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 120 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
