TThe ITK activity was detected using HTRF technology. The reaction was performed by incubating the ITK protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
ITK GST Tag Protein,Human
ITK GST Tag Protein,Human
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$720 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | EMT, LYK |
| Accession | Q08881 |
| Amino Acid Sequence | N2-L620(end); N-terminal GST tag |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 97.5 kDa |
| Purity | > 85% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
