ISG15 Recombinant Rabbit mAb (S-5475)

Catalog Number: S0B60359 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	ISG15
Synonyms	Ubiquitin-like protein ISG15; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein (IP17); Ubiquitin cross-reactive protein (hUCRP); G1P2; UCRP
Location	Cytoplasm, Secreted
Accession	P05161
Clone Number	S-5475
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu
IHC-P	1:500	Hu

Background

ISG15 (Interferon-Stimulated Gene 15) is a ubiquitin-like protein that is strongly induced by type I interferons and plays a critical role in innate immunity through a post-translational modification process known as ISGylation, which involves the covalent conjugation of ISG15 to target proteins via an enzymatic cascade comprising E1 (UBE1L), E2 (UBCH8), and E3 ligases such as HERC5. Functionally, ISG15 acts as a broad-spectrum antiviral effector by modifying viral or host proteins to inhibit viral replication, modulate signaling pathways like NF-κB and IRF3, and regulate immune cell activity, while also serving extracellularly as a cytokine-like molecule that stimulates IFN-γ production from T cells and NK cells upon binding to receptors such as LFA-1; conversely, its dysregulation is implicated in various pathologies including cancer, neurodegeneration, and autoinflammatory diseases, making it both a key biomarker for interferon signatures and a potential therapeutic target for modulating immune responses.

Picture

Western Blot

WB result of ISG15 Recombinant Rabbit mAb
Primary antibody: ISG15 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with hIFN-α1 (100ng/ml, 24 hours) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 17 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human spleen. Anti-ISG15 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ISG15 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human squamous cell carcinoma. Anti-ISG15 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.