WB result of IRGQ Recombinant Rabbit mAb
Primary antibody: IRGQ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RD whole cell lysate 20 µg
Lane 2: SK-BR-3 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: BxPC-3 whole cell lysate 20 µg
Lane 5: U-2 OS whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 65 kDa
IRGQ Recombinant Rabbit mAb (S-2648-17)
IRGQ Recombinant Rabbit mAb (S-2648-17)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IRGQ |
| Synonyms | Immunity-related GTPase family Q protein; IRGQ1 |
| Immunogen | Synthetic Peptide |
| Location | Lysosome |
| Accession | Q8WZA9 |
| Clone Number | S-2648-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | RD, SK-BR-3, MCF7, BxPC-3, U-2 OS, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
| IHC-P | 1:200 | Hu, Ms |
Background
IRGQ (Immunity-related GTPase family Q protein) is a newly recognized autophagy receptor that orchestrates quality control of MHC class I molecules by binding to the autophagy proteins GABARAPL2 and LC3B, thereby routing misfolded MHC-I heavy chains to lysosomal degradation; its absence allows these defective chains to accumulate and reach the cell surface, enhancing CD8⁺ T cell responses and improving survival in hepatocellular carcinoma models, and IRGQ is also implicated in Alzheime’s pathogenesis and exhibits broad cytoplasmic/nuclear expression across human tissues.
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Western Blot
WB result of IRGQ Recombinant Rabbit mAb
Primary antibody: IRGQ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 65 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-IRGQ antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-IRGQ antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-IRGQ antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-IRGQ antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
