WB result of IRF3 Recombinant Rabbit mAb
Primary antibody: IRF3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Lane 5: U937 whole cell lysate 20 µg
Lane 6: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 54 kDa
IRF3 Recombinant Rabbit mAb (S-3650-63)
IRF3 Recombinant Rabbit mAb (S-3650-63)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IRF3 |
| Synonyms | Interferon regulatory factor 3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Mitochondrion |
| Accession | Q14653 |
| Clone Number | S-3650-63 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms |
| Positive Sample | MCF7, THP-1, Daudi, A549, U937, Jurkat, NIH/3T3, RAW264.7, mouse skeletal muscle, mouse spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:500 | Hu |
Background
IRF3 (Interferon Regulatory Factor 3) is a constitutively expressed ~55 kDa transcription factor encoded by the IRF3 gene on chromosome 19, present in the cytoplasm of all tissues. Its N-terminal DNA-binding domain (DBD) contains five characteristic tryptophan repeats, while the C-terminus harbors a transcriptional activation domain tightly auto-inhibited by an intramolecular interaction—in the resting state, residues 380–427 at the C-terminus bind to residues 98–240 at the N-terminus, forming a "closed" conformation that blocks DNA-binding capacity. Upon sensing viral nucleic acids by pattern recognition receptors such as RIG-I/MDA5 or TLR3/4, upstream kinases—primarily TBK1/IKKε (or PKA in certain tissues)—phosphorylate multiple serine/threonine residues within the IRF3 C-terminus (notably the S385/S386 and 396–405 clusters). This phosphorylation triggers a conformational switch, exposing a hydrophobic core and promoting homodimerization. The activated dimers translocate to the nucleus, where they associate with CBP/p300 to form the DRAF1 complex, bind to ISRE elements in target gene promoters, and initiate immediate-early transcription of antiviral and chemotactic genes such as IFN-β and RANTES. This transcriptional activation is precisely restrained by the E3 ligase RAD18, which specifically recognizes DNA-bound, phosphorylated IRF3 dimers and catalyzes K63-linked ubiquitination at Lys193, causing IRF3 to dissociate from the IFNB1 promoter and undergo clearance via the autophagy-lysosome pathway, thereby preventing excessive interferon production and associated autoimmune pathology. More exquisitely, IRF3 exhibits non-transcriptional, dual intracellular functions: on one hand, early during viral infection, it directly binds the pro-apoptotic protein Bax via its BH3-like domain, inducing Bax conformational activation and co-translocation to mitochondria, thereby triggering IFN-independent apoptosis—a mechanism that eliminates viral replication factories and curbs persistent infection. On the other hand, under non-infectious hypoxic stress, cytoplasm-retained IRF3 directly engages HIF-1/2α and impedes their nuclear translocation, thereby suppressing hypoxic signaling and metabolic reprogramming—a discovery that unveiled an evolutionarily conserved, novel function of IRF3 in environmental adaptation beyond antiviral defense. Furthermore, in sterile inflammation such as liver fibrosis, IRF3 is phosphorylated by the Gαs-coupled GPCR-cAMP-PKA axis, driving IL-33 release and mediating hepatic stellate cell activation. This multifunctionality—serving as a transcription factor, apoptosis executor, hypoxia signaling suppressor, and metabolic nexus—positions IRF3 far beyond classical antiviral immunity, establishing it as a central intersection point linking infection, cell death, metabolic adaptation, and tissue fibrosis.
Picture
Picture
Western Blot
WB result of IRF3 Recombinant Rabbit mAb
Primary antibody: IRF3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Raw 264.7 whole cell lysate 20 µg
Lane 3: mouse skeletal muscle lysate 20 µg
Lane 4: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 42 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human placenta. Anti-IRF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-IRF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-IRF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-IRF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-IRF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-IRF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-IRF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
