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IP3R Recombinant Rabbit mAb

IP3R Recombinant Rabbit mAb

Catalog Number: S0B60796 Application: WB, IHC-P Reactivity: Ms, Rt Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen IP3R
Immunogen Synthetic Peptide
Location Cytoplasm, Endoplasmic reticulum
Accession P11881
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Ms, Rt
Positive Sample Mouse brain, rat brain, mouse cerebellum, rat cerebellum
Purification Protein A
Concentration 0.4 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Ms, Rt
IHC-P 1:500-1:2000 Ms, Rt

Background

IP3R (inositol 1,4,5-trisphosphate receptor) is a ligand-gated calcium release channel located on the endoplasmic reticulum membrane, with three isoforms—IP3R1, IP3R2, and IP3R3—encoded by the ITPR1, ITPR2, and ITPR3 genes, respectively; each subunit contains approximately 2,700 amino acids, and the functional channel is formed as a homotetramer. Its activation mechanism begins with extracellular signals (such as hormones, neurotransmitters, or growth factors) activating phospholipase C, which hydrolyzes PIP2 to generate IP3; IP3 binds to the N-terminal ligand-binding core domain of IP3R and, together with Ca²⁺, triggers channel opening, promoting the release of Ca²⁺ from endoplasmic reticulum stores into the cytoplasm and thereby regulating diverse physiological processes such as muscle contraction, synaptic transmission, learning and memory, cell proliferation, and apoptosis. The three isoforms differ in tissue distribution, affinity for IP3, and Ca²⁺ sensitivity: IP3R1 is highly expressed in neurons, IP3R2 is enriched in cardiomyocytes and hepatocytes, and IP3R3 is involved in tumors, exocrine secretion, and immune-related pathology. Mutations in ITPR1 are closely associated with spinocerebellar ataxia type 29, Gillespie syndrome, and congenital non-progressive ataxia.

Picture

Western Blot

WB result of IP3R Recombinant Rabbit mAb
Primary antibody: IP3R Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: unboiled mouse brain lysate 25 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 313 kDa
Observed MW: 300 kDa
This blot was developed with high sensitivity substrate

WB result of IP3R Recombinant Rabbit mAb
Primary antibody: IP3R Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: unboiled rat brain lysate 25 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 313 kDa
Observed MW: 300 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-IP3R antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-IP3R antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.