Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling mouse IgG2a (LALA-PG) isotype control at 1/500 (1 μg) dilution (Left) / (Red) compared with CD13 antibody at 1/2000 (0.1 μg) dilution (S0B0441) (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with mouse IgG2a (LALA-PG) isotype control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Invivo mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
Invivo mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Immunogen | Hen egg lysozyme (HEL) |
| Clone Number | S-R552 |
| Antibody Type | Mouse mAb |
| Isotype | Mouse IgG2a, κ |
| Mutations | LALA-PG |
| Application | In vivo control |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Endotoxin | <2EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Dilution
| application | dilution | species |
| FCM | 1:500 |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
Picture
Picture
FC
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling mouse IgG2a (LALA-PG) isotype control at 1/500 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with mouse IgG2a (LALA-PG) isotype control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody
