Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with Human IgG1 isotype control L234A,L235A,P329G (LALA-PG) antibody at 1/500 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Human IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Invivo Human IgG1 isotype control L234A,L235A,P329G (LALA-PG)
Invivo Human IgG1 isotype control L234A,L235A,P329G (LALA-PG)
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Product Details
Product Details
Product Specification
| Host | Human |
| Clone Number | S-5114 |
| Antibody Type | Recombinant mAb |
| Isotype | Human IgG1 |
| Application | FCM, In vivo control, ICFCM |
| Purification | Protein A |
| Concentration | 5 mg/ml |
| Purity | >95% (Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
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Flow cytometric analysis of C57BL/6 mouse splenocytes fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Human IgG1 isotype control L234A,L235A,P329G (LALA-PG) antibody at 1/500 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Human IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
