WB result of Invivo anti-Mouse VEGF-A Recombinant mAb
Primary antibody: Invivo anti-Mouse VEGF-A Recombinant mAb at 1/1000 dilution
Lane 1: mouse VEGF-A protein 2 µg
Secondary antibody: Goat Anti- rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 25-35 kDa
Invivo anti-Mouse VEGF-A Recombinant mAb
Invivo anti-Mouse VEGF-A Recombinant mAb
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$175 USD
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | VEGF-A |
| Synonyms | Vascular endothelial growth factor A, long form; L-VEGF; Vascular permeability factor (VPF); Vegf; Vegfa |
| Location | Secreted |
| Accession | Q00731 |
| Clone Number | S-2820 |
| Antibody Type | Rat mAb |
| Isotype | Rat IgG2a,k |
| Application | ELISA, WB, in vivo VEGF-A neutralization |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <2EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
Background
VEGF-A (Vascular Endothelial Growth Factor A) is a critical signaling protein that plays a central role in angiogenesis—the formation of new blood vessels from existing ones—by stimulating endothelial cell proliferation, migration, and vascular permeability, primarily through binding to its receptors VEGFR-1 and VEGFR-2 on endothelial cells, and is essential for normal physiological processes such as wound healing and embryonic development, while its dysregulation is implicated in pathological conditions including cancer, diabetic retinopathy, and age-related macular degeneration.
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Western Blot
