Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with anti - Mouse Siglec-H antibody at 1/500 dilution (1 μg) / (right panel) compared with a Rat IgG2b Isotype Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - Pacific Blue™ antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Invivo anti-Mouse Siglec-H Recombinant mAb
Invivo anti-Mouse Siglec-H Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Siglec-H |
| Synonyms | Siglec-H; Siglech |
| Location | Membrane |
| Accession | Q3Y597 |
| Clone Number | 440c |
| Antibody Type | Rat mAb |
| Isotype | Rat IgG2b, κ |
| Isotype Control | Invivo rat IgG2b isotype control, anti-keyhole limpet hemocyanin |
| Application | FCM, Functional assays |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95% (Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| FCM | 1:500 | Ms |
Background
Siglec-H is a sialic acid-binding Ig-like lectin expressed almost exclusively on murine plasmacytoid dendritic cells (pDCs) and microglia, where it partners with the ITAM-bearing adaptor DAP12 to deliver inhibitory signals that restrain type I interferon (IFN-α) secretion, limits TLR7/9-mediated viral responses, and thereby prevents overt systemic autoimmunity while still permitting antigen uptake and presentation; genetic deletion or antibody-mediated ligation of Siglec-H heightens IFN-α output, skews T-cell polarization toward IL-21⁺ CD4 subsets, and can either exacerbate or attenuate inflammatory diseases depending on context, making it a key rheostat of innate–adaptive immune crosstalk.
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