WB result of Invivo Anti-Mouse Myeloperoxidase (MPO) Recombinant mAb
Primary antibody: Invivo Invivo Anti-Mouse Myeloperoxidase (MPO) Recombinant mAb at 1/1000 dilution
Lane 1: MPO His Tag protein, Mouse 1 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate
Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Myeloperoxidase (MPO) |
| Synonyms | Mpo |
| Location | Lysosome |
| Accession | P11247 |
| Clone Number | S-3295 |
| Antibody Type | Recombinant mAb |
| Application | ELISA, WB, in vivo administration, in vitro administration |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, pH 7.4, Contains no stabilizers or preservatives |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| WB | 1:500 | Ms |
| FCM | 5μl per million cells in 100μl volume |
Background
Myeloperoxidase (MPO) is a ~150 kDa heme-containing glycoprotein stored in the azurophilic granules of neutrophils and monocytes that, upon degranulation, generates potent hypohalous acids (especially hypochlorous acid from H2O2 and Cl⁻) and reactive radical species to kill engulfed pathogens, but when released extracellularly it can oxidatively damage host lipids, proteins and DNA, thereby linking innate immunity to chronic inflammatory, cardiovascular and neurodegenerative diseases.
Picture
Picture
Western Blot
