Flow cytometric analysis of BALB/c mouse bone marrow labeled with Anti-Mouse Ly6G/Ly6C antibody at 1/500 dilution (1 μg) / (right panel) compared with a Rat IgG2b, κ Isotype Control / (left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD11b - Alexa Fluor® 647 antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Invivo Anti-Mouse Ly6G/Ly6C Recombinant mAb
Invivo Anti-Mouse Ly6G/Ly6C Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Ly6G/Ly6C |
| Synonyms | Lymphocyte antigen 6G; Ly-6G; Ly-6G.1; Ly6g |
| Location | Cell membrane |
| Accession | P35461 |
| Clone Number | S-5481 |
| Antibody Type | Rat mAb |
| Isotype | Rat IgG2b, κ |
| Isotype Control | S0B0997 |
| Application | FCM, in vivo cell specific depletion |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse bone marrow |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95% (Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| FCM | 1:500 | Ms |
Background
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein that belongs to the Ly-6 superfamily and is expressed in a developmentally regulated manner by myeloid cells, including neutrophils, granulocytes, and some monocytes. It serves as a key marker for mature neutrophils and is widely used in research to identify and deplete these cells in vivo. Ly6G is transiently expressed on monocytes during bone marrow development, while its expression on granulocytes and peripheral neutrophils correlates with their differentiation and maturation levels. This antigen plays important roles in immune responses, such as antitumor immunity and neutrophil-mediated inflammation.
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