WB result of Invivo Anti-Mouse IL-10 Recombinant mAb
Primary antibody: Invivo Anti-Mouse IL-10 Recombinant mAb at 1/1000 dilution
Lane 1: IL-10 Protein, Human 1 µg
Lane 2: IL-10 Protein, Mouse 1 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 17-27 kDa
Invivo Anti-Mouse IL-10 Recombinant mAb
Invivo Anti-Mouse IL-10 Recombinant mAb
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$120 USD
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$120 USD
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IL-10 |
| Synonyms | Interleukin-10; Cytokine synthesis inhibitory factor (CSIF); Il-10; Il10 |
| Location | Secreted |
| Accession | P18893 |
| Clone Number | S-3708 |
| Antibody Type | Recombinant mAb |
| Isotype | Rat IgG1,k |
| Application | ELISA, WB, in vivo lL-10 neutralization, in vitro lL-10 neutralization |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, pH 7.4, Contains no stabilizers or preservatives |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Ms |
Background
Interleukin-10 (IL-10) is an 18 kDa anti-inflammatory cytokine composed of 160 amino acids folded into two intrachain disulfide-linked α-helical dimers that signals through the heterotetrameric IL-10R1/IL-10R2 receptor complex to activate JAK1/TYK2-mediated STAT3 phosphorylation, thereby down-regulating major histocompatibility class II, co-stimulatory molecules and pro-inflammatory cytokines such as TNF-α, IL-1, IL-6, IL-8, IL-12 and IFN-γ, while simultaneously up-regulating IL-1RA and soluble TNF receptors, ultimately exerting potent immunosuppressive and tissue-protective functions in both innate and adaptive immunity.
Picture
Picture
Western Blot
