Flow cytometric analysis of C57BL/6 mouse splenocytes, treated 3 days with 500 ng/ml LPS (right panel) or untreated (left panel), labeled with Mouse CD80 (B7-1) at 1/500 dilution (1 μg). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Invivo anti-Mouse CD80 (B7-1) Recombinant mAb
Invivo anti-Mouse CD80 (B7-1) Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD80 |
| Synonyms | T-lymphocyte activation antigen CD80; Activation B7-1 antigen (B7); B7; Cd80 |
| Location | Cell membrane |
| Accession | Q00609 |
| Clone Number | 1G10 |
| Antibody Type | Rat mAb |
| Isotype | Mouse IgG2a, κ |
| Isotype Control | S0B0932 |
| Application | in vivo blocking, in vitro blocking, FCM |
| Reactivity | Ms |
| Positive Sample | LPS treated C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 5 mg/ml |
| Purity | >95% (Determined by SDS-PAGE) |
| Endotoxin | <1EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage | 2 to 8 °C for 2 weeks under sterile conditions; |
Dilution
| application | dilution | species |
| FCM | 1: 500 | Ms |
Background
CD80，also known as B7-1，is a co - stimulatory molecule expressed on antigen - presenting cells（APCs）such as dendritic cells，macrophages and B cells. It plays a crucial role in the immune response by interacting with CD28 on T cells to provide a co - stimulatory signal necessary for T cell activation，thereby promoting T cell proliferation and differentiation. Additionally，CD80 can also bind to CTLA - 4，an inhibitory receptor on T cells，which acts as a negative regulator of T cell activation. This binding can inhibit the immune response and maintain immune tolerance. Moreover，CD80 - CD28 interaction is involved in the regulation of various immune - related processes，including the formation of immunological synapses and the regulation of cytokine production.
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