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Invivo Anti-Mouse CD28 Recombinant mAb

Invivo Anti-Mouse CD28 Recombinant mAb

Catalog Number: S0B7188 Application: ELISA, FCM, in vivo cell specific stimulation/activation, in vitro cell specific stimulation/activation Reactivity: Ms Conjugation: Unconjugated Brand: Starter
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$175 USD
$175 USD
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Product Details

Product Specification


Host Mouse
Antigen CD28
Location Cell membrane
Accession P31041
Clone Number S-5510
Antibody Type Mouse mAb
Isotype Mouse IgG1, κ
Isotype Control S0B0760
Application ELISA, FCM, in vivo cell specific stimulation/activation, in vitro cell specific stimulation/activation
Reactivity Ms
Positive Sample C57BL/6 mouse splenocytes
Purification Protein A
Concentration 5 mg/ml
Purity >95% (Determined by SDS-PAGE)
Endotoxin <1EU/mg
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS pH7.4, containing no preservative
Stability & Storage

2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.

Dilution


application dilution species
FCM 1:500 Ms

Background

CD28, a 44-kDa disulfide-linked glycoprotein, is constitutively expressed on the surface of most CD4+ and CD8+ T cells. It plays a crucial role in T cell activation and signaling by interacting with its ligands CD80 (B7.1) and CD86 (B7.2) on antigen-presenting cells. CD28 enhances T cell receptor signaling, promotes cell cycle entry, and supports the survival of T cells by upregulating anti-apoptotic proteins like Bcl-2. It also preferentially promotes TH2 differentiation, aiding B cell germinal center formation and isotype switching. CD28 signaling involves multiple pathways, including PI3K, Grb2, and NFκB activation, which collectively regulate T cell proliferation, differentiation, and effector functions.

Picture

FC

Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD28 antibody at 1/500 dilution (1 μg) / (right panel) compared with a Mouse IgG1, κ Isotype Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ antibody separately. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.